LAMP or loop-mediated amplification method is an isothermal nucleic acid amplification. LAMP is isothermal which eradicates the need for expensive thermal cyclers used in conventional PCR.
LAMP is a relatively new DNA amplification technique, which, due to its simplicity, ruggedness, and low cost, could provide major advantages. In LAMP, the target sequence is amplified at a constant temperature of 60 – 65 °C using together three sets of primers and a polymerase with high strand displacement activity in addition to a replication activity. Typically, 6 different primers are used to identify 6 distinct regions on the target gene, which adds specificity. Due to the specific nature of the action of these primers, the amount of DNA produced in LAMP is considerably higher than amplification based on PCR. The corresponding release of pyrophosphate results in visible turbidity due to precipitation. Detection of homozygous wild-type, heterozygous and homozygous mutant alleles is performed by fluorophore-labelled probe-based melting curve analysis.