LAMP Human HLA Coeliac Direct KIT
The LAMP Human HLA Coeliac Direct KIT is an in vitro diagnostic test intended for the qualitative detection of DQA1*05, DQB1*02 and DQB1*0302 alleles by Loop-mediated isothermal amplification (LAMP) on EDTA whole blood and extracted DNA. This assay is dedicated to professional use in diagnostic laboratories. The device is not for self-testing.
Regulatory status: CE-IVD
Format: 24 / 96 reactions
Linked disease: Celiac disease
Alleles: DQA1*05, DQB1*02 and DQB1*0302
Method: LAMP + meting curve analysis
Sample: EDTA whole blood - no DNA extraction required - or extracted DNA
Compatible instruments: LightCycler 480 I&II / Cobas z 480 (Roche); CFX96 / CFX Opus 96 (Bio-Rad); QuantStudio 1/3/5 & 6/7 Flex (Applied Biosystems); MIC qPCR (Bio Molecular Systems); LC-Genie III & Genie HT (Optigene)
Software / result interpretation: Automated genotype calling via GeneFox software; mandatory visual confirmation of the melting curve
Turn around time: < 1h
General information
Advantages
Linked products
Coeliac Disease (CD) is an autoimmune disease characterized by an inflammation of the small intestine due to ingestion of gluten.
Human leukocyte antigen (HLA) genotyping can be an aid in diagnosis based on the exceptionally strong association of CD with specific HLA genotypes: HLA-DQ2.5 haplotype (alleles DQA1*05 and DQB1*02), HLA-DQ2.2 (alleles DQA1*0201 and DQB1*02), HLA-DQ8 (alleles DQA1*0301 and DQB1*0302) and/or HLA-DQ7 (DQA1*05).
Moreover, testing for these haplotypes has a high negative predicting value as most people negative for these alleles do not develop CD.
Background Information
You have a question regarding our products ? You want to know more about our method ?
Find out about our other expertises
