LAMP Human Lactose intolerance Duplex KIT
The LAMP Human Lactose Intolerance Duplex KIT is an in vitro diagnostic test intended for the qualitative detection of the MCM6 polymorphisms, -13910 C/T (rs4988235), -13915 T/G (rs41380347), -14010 G/C (rs145946881) and -14009 T/G (rs869051967), by Loop-mediated isothermal amplification (LAMP) on EDTA whole blood and extracted DNA. This assay is dedicated to professional use in diagnostic laboratories. The device is not for self-testing.
Regulatory status: CE-IVD
Format: 96 reactions
Target gene: LCT gene
Linked disease: Lactose intolerance
Mutation: 13910 C/T polymorphism, -13915 T/G polymorphism, -14010 G/C polymorphism, -14009 T/G polymorphism and surrounding mutations
Method: LAMP + meting curve analysis
Sample: EDTA whole blood - no DNA extraction required - or extracted DNA
Compatible instruments: LightCycler 480 I&II / Cobas z 480 (Roche); MIC qPCR (Bio Molecular Systems)
Software / result interpretation: Automated genotype calling via GeneFox software; mandatory visual confirmation of the melting curve
Turn around time: < 1h
General information
Advantages
Linked products
Lactose intolerance in adulthood is caused by reduced production of lactase after infancy, called lactase non-persistence or adult-type hypolactasia and is the most common type of lactose intolerance. The expression of the enzyme lactase, encoded by the LCT gene, is regulated by a DNA sequence located nearby, the MCM6 gene. Changes in this element have led to sustained lactase production in the small intestine and the ability to digest lactose throughout life. People without these changes have a reduced ability to digest lactose as they get older, resulting in the signs and symptoms of lactose intolerance.
Background Information
You have a question regarding our products ? You want to know more about our method ?
Find out about our other expertises
