LAMP Human FV LEIDEN KIT
The LAMP Human FV LEIDEN mutation KIT is an in vitro diagnostic test intended for the qualitative detection of Factor V Leiden G1691A by Loop-mediated isothermal amplification (LAMP) on EDTA whole blood and extracted DNA. This assay is dedicated to professional use in diagnostic laboratories. The device is not for self-testing.
Format: 24 / 96 reactions
Mutation: c.1601G>A
Method: LAMP + meting curve analysis
Sample: EDTA whole blood - no DNA extraction required - or extracted DNA
Compatible instruments: LightCycler 480 I&II / Cobas z 480 (Roche); CFX96 / CFX Opus 96 (Bio-Rad); QuantStudio 1/3/5 & 6/7 Flex (Applied Biosystems); MIC qPCR (Bio Molecular Systems); LC-Genie III & Genie HT (Optigene)
Software / result interpretation: Automated genotype calling via GeneFox software; mandatory visual confirmation of the melting curve
Turn around time: < 1h
General information
Advantages
Linked products
The LC-FVL-LP kit uses loop mediated isothermal amplification, a robust amplification method using four to six primers per target, that can be directly used on lysed EDTA-whole blood samples, without DNA purification and on extracted DNA samples from whole blood samples.
The genotyping is performed by melting curve analysis after amplification, using a specific probe and quencher for each target SNP.
Test principle
Factor V Leiden is one of the primary genetic causes for inherited thrombophilia, a blood coagulation disorder.
Factor V Leiden thrombophilia is characterized by a poor anticoagulant response to activated protein C (APC) and an increased risk for venous thromboembolism (VTE). The allele frequency of Factor V Leiden polymorphism is 3-8% in the Caucasian population.
Background Information
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