LAMP Human HLA-B27 direct detection KIT
The LAMP Human HLA-B27 Direct KIT is an in vitro diagnostic test intended for the qualitative detection of most HLA-B27 alleles, included European most AS associated subtypes B*2702 and B*2705, Asian subtype B*2704 and African subtype B*2703 by Loop-mediated isothermal amplification (LAMP) on EDTA whole blood and extracted DNA.
This assay is dedicated to professional use in diagnostic laboratories.The device is not for self-testing.
Regulatory Status: CE-IVD
Format: 24 / 96 reactions
Target gene: HLA-B27
Linked disease: Ankylosins spondylitis (AS)
Alleles: B*2702 and B*2705, Asian subtype B*2704 and African subtype B*2703.
Method: LAMP + meting curve analysis
Sample: EDTA whole blood - no DNA extraction required - or extracted DNA
Compatible instruments: LightCycler 480 I&II / Cobas z 480 (Roche); CFX96 / CFX Opus 96 (Bio-Rad); QuantStudio 1/3/5 & 6/7 Flex (Applied Biosystems); MIC qPCR (Bio Molecular Systems); LC-Genie III & Genie HT (Optigene)
Software / result interpretation: Automated genotype calling via GeneFox software; mandatory visual confirmation of the melting curve
Turn around time: < 1h
General information
Advantages
Linked products
Ankylosing spondylitis (AS) is a common, highly heritable inflammatory arthritis affecting primarily the spine and pelvis (Hadler et al., 2013) and affects approximatively 0.5% of the population (Shaikh, 2007).
Accumulating evidence has demonstrated that human leukocyte antigen (HLA) class I molecule B27 (HLA-B27) is strongly associated to AS (Lin & Gong, 2017) : > 90 % of AS patients are HLA-B27 positive (Bowness, 2015)(Oliveira, 2016).
Over 100 different allelic variants of B27 have been recognized (HLA-B2701 to HLA-B27106) in AS patients, among which B27*05 and B27*02 are widely prevalent in European populations, and B27*04 is predominate in Asian populations (Lin & Gong, 2017).
Background Information
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