LAMP Human PAI-1 mutation KIT
The LAMP Human PAI-1 mutation KIT is an in vitro diagnostic test intended for the qualitative detection of PAI-1 insertion/deletion (5G/4G) by Loop-mediated isothermal amplification (LAMP) on EDTA whole blood and extracted DNA. This assay is dedicated to professional use in diagnostic laboratories. The device is not for self-testing.
Regulatory Status: CE-IVD
Format: 24 reactions
Target gene: PAI-1
Linked disease: Thrombophilia
Method: LAMP + meting curve analysis
Sample: EDTA whole blood - no DNA extraction required - or extracted DNA
Compatible instruments: LightCycler 480 I&II / Cobas z 480 (Roche); CFX96 / CFX Opus 96 (Bio-Rad); QuantStudio 1/3/5 & 6/7 Flex (Applied Biosystems); MIC qPCR (Bio Molecular Systems); LC-Genie III & Genie HT (Optigene)
Software / result interpretation: Automated genotype calling via GeneFox software; mandatory visual confirmation of the melting curve
Turn around time: < 1h
General information
Advantages
Linked products
The assay uses loop mediated isothermal amplification, a robust amplification method using four to six primers per target, that can be directly used on lysed EDTA-whole blood sample, without DNA purification and on extracted DNA samples from whole blood samples.
The genotyping is performed by melting curve analysis after amplification, using a specific probe and quencher for each target SNP.
Test principle
A common single nucleotide insertion/deletion (5G/4G) in the promotor region of the plasminogen activator inhibitor-1 (PAI-1) gene results in higher levels of PAI-1 in the serum. Since PAI-1 indirectly inhibits the breakdown of blood clots, heterozygous or homozygous carriers of this mutation have an increased risk of venous thrombosis, particularly when other relevant thrombosis-related mutations are present simultaneously.
Background Information
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