LAMP Human DPD Deficiency KIT - DPYD*6

The assay with the “LAMP Human DPD Deficiency KIT-DPYD*6” is performed on whole blood samples either freshly collected or stored at -20°C, or on extracted DNA samples.

The “LAMP Human DPD Deficiency KIT-DPYD*6” contains 6 specific primers allowing the loop-mediated amplification of a specific region surrounding the polymorphism. Each amplified target sequence is detected by a probe through the detection of fluorescence variation. After amplification, the temperature is decreased to 40°C and the probe hybridizes the amplified fragment, bringing the fluorophore and the quencher in proximity, resulting in quenching of the fluorescence. During the melting curve analysis, the temperature is gradually increased while the change in fluorescence emission is measured. The probe will detach differently from amplified wild type DNA fragments and mutated DNA fragments, therefore, the change in fluorescence will be observed at a different temperature, allowing to make the difference between homozygous mutated, heterozygous mutated or wild type for the DPYD*6 allele.